Alok Raghav and Jamal Ahmad

¹Multidiscplinary Research Unit, GSVM Medical College, Kanpur, India-208002

²Rajiv Gandhi Centre for Diabetes and Endocrinology, Aligarh Muslim University, Aligarh-India-202001

Abstract

Objective:

This study investigates the role of Chronic unpredictable environmental stress (CUES) on impaired homeostasis

Methods:

Stressed group mice (n = 20) were exposed to CUES for 16 weeks. Weekly body weight, feed consumption, feed efficiency ratio, fasting blood glucose were monitored. Plasma HbA1c, plasma cortisol, plasma epinephrine and plasma insulin, serum lipids, antioxidants and carbohydrate metabolizing enzymes activity were assessed along with DNA damage and histopathological examination of liver, kidney, pancreas, spleen and skeletal muscles

Results:

Fasting blood glucose levels & HbA1c in the stressed were significantly higher compared to control (p < 0.001). Serum lipids were found insignificantly higher in stressed mice compared to control. Body weights of the stressed mice and feed efficiency ratio were found significant (p < 0.001). Plasma corticosterone, plasma epinephrine, HOMA-IR was found to be significantly higher in the stressed group (p < 0.001). Plasma insulin level was found to be significantly lower in the stressed group (p < 0.001). Significant changes were observed in antioxidants level, carbohydrate metabolizing enzymes activity, peripheral tissues and DNA integrity.

Conclusion:

Chronic unpredictable environmental stress initiates pathogenesis of diabetes.